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Proteomics facility added at NIO
NIO at its Headquarters in Goa enriched its biological laboratories by adding a new proteomics facility. The instruments under this facility include LCMS QToF (Liquid Chromotography Mass Spectrometer Quadrapole Time of Flight), 2-D gel electrophoresis unit, Off-gel electrophoresis unit and BioAnalyzer (chip electrophoresis).
LCMS QToF is capable of analysing multiple types of molecules that could be ionised. The instrument is being used for Proteomics, under which, both qualitative and quantitative analysis of proteins is carried out. The CHIP nanoLC technology has both enrichment (40 nL) and analytical (200 nL) columns on a chip, and uses minimal volumes of samples for separation and detection, in place of a conventional LC for separation of proteins. With this, a complex mixture of proteins e.g. cell lysate, could be resolved and detected in as little as 30 minutes.
The MS has sensitivity up to femtogram which level helps detecting proteins at lower concentrations. Both gel-based (SDS PAGE and 2-D PAGE) and in-solution digested proteins are identified using this set-up.
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